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gene chip hybridization oven 645  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene chip hybridization oven 645
    Gene Chip Hybridization Oven 645, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+chip+hybridization+oven/bio_rxiv__2023__05__17__540900-231-22-27
    Average 90 stars, based on 1 article reviews
    gene chip hybridization oven 645 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis
    Article Snippet: Fragmentation and labeling were performed using the Affymetrix Gene Chip WT Terminal Labeling and Hybridization Kit. .. The enzymatically fragmented and end labeled ssDNAs were hybridized to Affymetrix Bovine Gene 1.0 ST Arrays for 16 h at 45 °C in an Affymetrix Gene Chip Hybridization Oven. .. The microarrays were scanned at a 0.7-μm resolution with the Affymetrix Gene Chip Scanner 3000 7G.

    Article Title: Omics underpins novel clues on VDR chemoprevention target in breast cancer.
    Article Snippet: Breast cancer is the commonest form of female malignancy among women in Western countries.. The advent of genomic technologies has enhanced the diagnosis and the biological classification of such pathology.. It has been demonstrated that cancer takes many years to be fully established.

    Article Title: Direct and delayed X-ray-induced DNA damage in male mouse germ cells.
    Article Snippet: The Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay was used to amplify and reverse-transcribe total RNA (300 ng) and to biotinylate sense-strand DNA targets. .. Arrays were then hybridized with the labeled-target hybridization cocktail by rotation at 60 rpm in the Affymetrix Gene Chip hybridization oven at 458C for 16 hr, washed in the Affymetrix GeneChip Fluidics station FS-450, and scanned by Affymetrix Gene Chip scanner 3000 7G system. .. Scanned image files (.CEL) were processed, normalized (RMA-Sketch Quantile), and Log2-transformed using the Expression Console Software (Affymetrix, http://www.affymetrix.com).

    Article Title: Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy
    Article Snippet: Briefly, the Affymetrix GeneChip® Whole Transcript Sense Target Labelling Assay was used to amplify and reverse-transcribe total RNA, and to biotinylate sense-strand DNA targets. .. Arrays were hybridized with the labeled target hybridization cocktail by rotation in the Affymetrix Gene Chip hybridization oven at 45°C for 16 h, washed in the Affymetrix GeneChip Fluidics station FS 450, and scanned by Affymetrix Gene Chip scanner 3000 7G system. .. siRNA The 22-nt siRNA duplexes corresponding to nucleotides 1062–1083 (siChe-1-1), 1473–1492 (siChe-1-2) and 2093–2111 (siChe-1-3) of human Che-1 sequence, and to nucleotides 122–143 of the negative control green fluorescent protein (GFP), were synthesized in vitro by using the Silencer siRNA construction kit (Ambion), following the manufacturer's instruction.

    Hybridization:

    Article Title: Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis
    Article Snippet: Fragmentation and labeling were performed using the Affymetrix Gene Chip WT Terminal Labeling and Hybridization Kit. .. The enzymatically fragmented and end labeled ssDNAs were hybridized to Affymetrix Bovine Gene 1.0 ST Arrays for 16 h at 45 °C in an Affymetrix Gene Chip Hybridization Oven. .. The microarrays were scanned at a 0.7-μm resolution with the Affymetrix Gene Chip Scanner 3000 7G.

    Article Title: Renal Cell Neoplasms Contain Shared Tumor Type-Specific Copy Number Variations
    Article Snippet: .. Samples were subsequently hybridized on Genome-Wide SNP 6.0 arrays for 18 hours at 50°C with rotation (60 rpm) in an Affymetrix Gene Chip Hybridization Oven (model 640). .. Arrays were washed, stained, and scanned according to the Affymetrix Genome-Wide SNP 6.0 protocol using the Affymetrix GeneChip Fluidics Station (model 450), GeneChip Scanner 3000 7G, and GeneChip Command Console software version 3.0.2 (Affymetrix, Sunnyvale, CA).

    Article Title: Integration ofERGgene mapping and gene-expression profiling identifies distinct categories of human prostate cancer
    Article Snippet: Sameer Jhavar 1 , Daniel Brewer 1 , Sandra Edwards 1 , Zsofia Kote-Jarai 1 , Gerhardt Attard , Jeremy Clark 1 , Penny Flohr 1 , Timothy Christmas 3 , Alan Thompson 3 , Matthew Parker 1 , Christopher Shepherd 1 , Ulf-Hakan Stenman 4 , Tania Marchbank 5 , Raymond J. Playford 5 , Christopher Woodhouse 3 , Christopher Ogden 3 , Cyril Fisher 6 , Gyula Kovacs 7 , Cathy Corbishley 8 , Charles Jameson 6 , Andy Norman 2 , Johann De-Bono 2 , Anders Bjartell 9 , Rosalind Eeles 1,2 and Colin S. Cooper 1

    Article Title: Omics underpins novel clues on VDR chemoprevention target in breast cancer.
    Article Snippet: Breast cancer is the commonest form of female malignancy among women in Western countries.. The advent of genomic technologies has enhanced the diagnosis and the biological classification of such pathology.. It has been demonstrated that cancer takes many years to be fully established.

    Article Title: Direct and delayed X-ray-induced DNA damage in male mouse germ cells.
    Article Snippet: The Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay was used to amplify and reverse-transcribe total RNA (300 ng) and to biotinylate sense-strand DNA targets. .. Arrays were then hybridized with the labeled-target hybridization cocktail by rotation at 60 rpm in the Affymetrix Gene Chip hybridization oven at 458C for 16 hr, washed in the Affymetrix GeneChip Fluidics station FS-450, and scanned by Affymetrix Gene Chip scanner 3000 7G system. .. Scanned image files (.CEL) were processed, normalized (RMA-Sketch Quantile), and Log2-transformed using the Expression Console Software (Affymetrix, http://www.affymetrix.com).

    Article Title: The effect of oxythioquinox exposure on normal human mammary epithelial cell gene expression: A microarray analysis study
    Article Snippet: Excess biotinylated dUTPs were removed by RNEasy Mini Kit before being fragmented and added to a hybridization cocktail, including Eukaryotic Hybridization controls (Affymetrix), BSA and herring sperm DNA (Gibco, Grand Island, NY) and biotinylated anti-streptavidin antibody (Vector Laboratories, Burlingame, CA). .. Hybridization on microarrays was performed for 16 hours at 45°C in the Gene Chip Hybridization Oven with rocker (Affymetrix). .. Microarrays were washed and stained using the protocol, as described in the Affymetrix Manual, with the GeneChip Fluidics Station 400 (Affymetrix).

    Article Title: Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy
    Article Snippet: Briefly, the Affymetrix GeneChip® Whole Transcript Sense Target Labelling Assay was used to amplify and reverse-transcribe total RNA, and to biotinylate sense-strand DNA targets. .. Arrays were hybridized with the labeled target hybridization cocktail by rotation in the Affymetrix Gene Chip hybridization oven at 45°C for 16 h, washed in the Affymetrix GeneChip Fluidics station FS 450, and scanned by Affymetrix Gene Chip scanner 3000 7G system. .. siRNA The 22-nt siRNA duplexes corresponding to nucleotides 1062–1083 (siChe-1-1), 1473–1492 (siChe-1-2) and 2093–2111 (siChe-1-3) of human Che-1 sequence, and to nucleotides 122–143 of the negative control green fluorescent protein (GFP), were synthesized in vitro by using the Silencer siRNA construction kit (Ambion), following the manufacturer's instruction.

    Genome Wide:

    Article Title: Renal Cell Neoplasms Contain Shared Tumor Type-Specific Copy Number Variations
    Article Snippet: .. Samples were subsequently hybridized on Genome-Wide SNP 6.0 arrays for 18 hours at 50°C with rotation (60 rpm) in an Affymetrix Gene Chip Hybridization Oven (model 640). .. Arrays were washed, stained, and scanned according to the Affymetrix Genome-Wide SNP 6.0 protocol using the Affymetrix GeneChip Fluidics Station (model 450), GeneChip Scanner 3000 7G, and GeneChip Command Console software version 3.0.2 (Affymetrix, Sunnyvale, CA).



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